Dynamic light scattering in protein crystallization: analysis and optimization

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Entrapment–D-(+)-Glucose Water Nanodroplet: Synthesis and Dynamic Light Scattering

In the present research, the D-(+)-Glucose–confined water nanodroplets with mean size in the range of 6-11 nm were synthesized at D-(+)-Glucose different concentrations by AOT reverse micelles (RMs) method as a function of mass fraction of droplet (MFD) at the constant water-to-surfactant molar ratio ( W=40). The dynamic light scattering of the nano-sized water droplets containing the D-(+)-Glu...

متن کامل

Systematic analysis of protein–detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials

Detergents are widely used for the isolation and solubilization of membrane proteins to support crystallization and structure determination. Detergents are amphiphilic molecules that form micelles once the characteristic critical micelle concentration (CMC) is achieved and can solubilize membrane proteins by the formation of micelles around them. The results are presented of a study of micelle ...

متن کامل

Monitoring and Scoring Counter-Diffusion Protein Crystallization Experiments in Capillaries by in situ Dynamic Light Scattering

In this paper, we demonstrate the feasibility of using in situ Dynamic Light Scattering (DLS) to monitor counter-diffusion crystallization experiments in capillaries. Firstly, we have validated the quality of the DLS signal in thin capillaries, which is comparable to that obtained in standard quartz cuvettes. Then, we have carried out DLS measurements of a counter-diffusion crystallization expe...

متن کامل

Effect of tocopherol on Pluronic microemulsions: turbidity studies and Dynamic light scattering and dynamic surface tension measurements

The development and design of the biocompatible and biodegradable thermodynamically stable micellar and microemulsion transparent dispersions to reduce the free and unbounded drugs concentration in the blood is a basic challenge in field of drug efficacy and bioavailability of drugs. In the current work, solubilization capacity of the drug (Tocopherol), oil (Ethyl Butyrate), and oil+drug (1:1 m...

متن کامل

Dynamic Light Scattering

A HeNe laser beam incident on a sample of micron-sized spheres suspended in water scatters into a random pattern of spots of varying size, shape, and intensity. The pattern results from the coherent superposition of the outgoing waves scattered from the spheres. Because the spheres are in constant Brownian motion, the pattern randomly changes in time. A photodetector is placed in the pattern an...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography

سال: 2008

ISSN: 0108-7673

DOI: 10.1107/s0108767308098449